LOCUS       pLV-[hsa-miR-20a]        9125 bp    DNA     circular     15-MAR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7046..7906
                     /label=Amp(R)
     promoter        8107..8647
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5651..5885
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6460..6915)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5957..6087
                     /label=SV40 pA
     misc_feature    6088..6301
                     /label=SV40 ori
     misc_feature    4972..5568
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3590
                     /label=intron
     CDS             3637..4962
                     /label=rPuro (RFP Puro)
     misc_feature    3381..3386
                     /label=XbaI/NheI
     misc_RNA        3110..3380
                     /label=hsa-mir-20a precursor
BASE COUNT     2156 a      2358 c      2464 g      2147 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct ggcctgctat 
     3121 ttccttcaaa tgaatgattt ttactaattt tgtgtacttt tattgtgtcg atgtagaatc 
     3181 tgcctggtct atctgatgtg acagcttctg tagcactaaa gtgcttatag tgcaggtagt 
     3241 gtttagttat ctactgcatt atgagcactt aaagtactgc tagctgtaga actccagctt 
     3301 cggcctgtcg cccaatcaaa ctgtcctgtt actgaacact gttctatggt tagttttgca 
     3361 ggtttgcatc cagctgtgtg tctagctcga gcttttggag tacgtcgtct ttaggttggg 
     3421 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc 
     3481 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt 
     3541 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa 
     3601 aactacccct ctagagtcga gctaccggtc gccaccatgg tgagcaaggg cgaggaggat 
     3661 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac 
     3721 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc 
     3781 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct 
     3841 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg 
     3901 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc 
     3961 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag 
     4021 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg 
     4081 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag 
     4141 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc 
     4201 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc 
     4261 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc 
     4321 ggcggcatgg acgagctgta caaggaccca ccggtcgcca ccatgaccga gtacaagccc 
     4381 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 
     4441 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 
     4501 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 
     4561 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 
     4621 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 
     4681 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 
     4741 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 
     4801 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 
     4861 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga gtgcccgaag 
     4921 gaccgcgcga cctggtgcat gacccgcaag cccggtgcct gagcggccgc gtcgacaatc 
     4981 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 
     5041 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 
     5101 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 
     5161 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 
     5221 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 
     5281 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 
     5341 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 
     5401 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 
     5461 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 
     5521 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc 
     5581 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 
     5641 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg 
     5701 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 
     5761 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 
     5821 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 
     5881 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 
     5941 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     6001 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     6061 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc 
     6121 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 
     6181 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 
     6241 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct 
     6301 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 
     6361 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 
     6421 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat 
     6481 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 
     6541 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 
     6601 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 
     6661 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 
     6721 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 
     6781 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 
     6841 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 
     6901 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 
     6961 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 
     7021 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 
     7081 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 
     7141 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 
     7201 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 
     7261 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 
     7321 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 
     7381 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 
     7441 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 
     7501 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 
     7561 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 
     7621 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 
     7681 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 
     7741 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 
     7801 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 
     7861 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 
     7921 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
     7981 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
     8041 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     8101 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     8161 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     8221 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     8281 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     8341 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     8401 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     8461 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     8521 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     8581 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     8641 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
     8701 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 
     8761 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 
     8821 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 
     8881 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 
     8941 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 
     9001 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 
     9061 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga 
     9121 gctgc 
//
